Glowing Grotto is a hard-difficulty track based in Mystic Jungle. It contains massive six-lane half-pipes and Grind Rails.
In Glowing Grotto, the playable characters are stuck running down the level's road and can only move left and right between the road's lanes (akin to the Quick Step). While running, the player can pick up powerups from Item Boxes and utilize the gimmicks native to this track.
Glowing Grotto has many environmental obstacles which the player can either jump over, roll under or move around. Running into one of these obstacles will result in the player taking damage and lose some of their Rings. Falling into bottomless pits on the other hand forces the player to restart from an earlier point on the track.
The racers alternate between running through alternating four and six-lane sections of either land or Grind Rails, the former of which has many Item Boxes. There are numerous obstacles such as electric trees placed in the racers' paths, and the stage has two sets of Triple Dash Pads positioned in the middle and near the end.
Along with the other Mystic Jungle tracks, Lambent Grove is one of the stages with a different song that plays during a race.